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anti cd45ra apc  (Miltenyi Biotec)


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    Miltenyi Biotec anti cd45ra apc
    Anti Cd45ra Apc, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/apc+anti+human+cd45ra/CD45RA+Antibody%2C+anti-human%2C+REAlease/pmc12221282-10-0-2
    Average 93 stars, based on 5 article reviews
    anti cd45ra apc - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Chromatin Immunoprecipitation:

    Article Title: Microbial peptides activate tumour-infiltrating lymphocytes in glioblastoma
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used PerCP/Cy5.5 anti-human CD3 antibody, HIT3a clone, Biolegend, cat.300328; APC-Cy7 anti-human CD4 antibody, OKT4 clone, Biolegend, cat. 317418; Anti-CD3 monoclonal antibody, OKT3 clone, ThermoFisher, cat. 16-0037-81; APC anti-human CD4, RPA-T4 clone, Biolegend, cat. 300514; Pacific blue anti-human CD8, SK1 clone, Biolegend, cat. 344718; APC anti-human CD45RA, HI100 clone, Biolegend, cat. 304112; PE-Cy7 anti-human HLA-DR, L243 clone, Biolegend, cat. 307616; CellTraceTM CFSE Cell Proliferation Kit, for flow cytometry, ThermoFisher, cat. C34554; CD45RA MicroBeads, human, Miltenyi Biotec, cat. 130-045-901; ELISA MAXTM Standard Set Human IFN-γ, Biolegend, cat. 430101; LEGENDplex Human T Helper Cytokine Panels, Biolegend, cat. 740001; LIVE/DEADTM Fixable Yellow Dead Cell Stain Kit, for 405 nm excitation, ThermoFisher, cat. L34968; LIVE/DEADTM Fixable Aqua Dead Cell Stain Kit, for 405 nm excitation, ThermoFisher, cat. L34957; Human GM-CSF DuoSet ELISA, rndsystems, cat. DY215; Human XL Cytokine Luminex® Performance Assay 44-plex Fixed Panel, rndsystems,cat. ..

    Flow Cytometry:

    Article Title: Microbial peptides activate tumour-infiltrating lymphocytes in glioblastoma
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used PerCP/Cy5.5 anti-human CD3 antibody, HIT3a clone, Biolegend, cat.300328; APC-Cy7 anti-human CD4 antibody, OKT4 clone, Biolegend, cat. 317418; Anti-CD3 monoclonal antibody, OKT3 clone, ThermoFisher, cat. 16-0037-81; APC anti-human CD4, RPA-T4 clone, Biolegend, cat. 300514; Pacific blue anti-human CD8, SK1 clone, Biolegend, cat. 344718; APC anti-human CD45RA, HI100 clone, Biolegend, cat. 304112; PE-Cy7 anti-human HLA-DR, L243 clone, Biolegend, cat. 307616; CellTraceTM CFSE Cell Proliferation Kit, for flow cytometry, ThermoFisher, cat. C34554; CD45RA MicroBeads, human, Miltenyi Biotec, cat. 130-045-901; ELISA MAXTM Standard Set Human IFN-γ, Biolegend, cat. 430101; LEGENDplex Human T Helper Cytokine Panels, Biolegend, cat. 740001; LIVE/DEADTM Fixable Yellow Dead Cell Stain Kit, for 405 nm excitation, ThermoFisher, cat. L34968; LIVE/DEADTM Fixable Aqua Dead Cell Stain Kit, for 405 nm excitation, ThermoFisher, cat. L34957; Human GM-CSF DuoSet ELISA, rndsystems, cat. DY215; Human XL Cytokine Luminex® Performance Assay 44-plex Fixed Panel, rndsystems,cat. ..

    Magnetic Resonance Imaging:

    Article Title: Microbial peptides activate tumour-infiltrating lymphocytes in glioblastoma
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used PerCP/Cy5.5 anti-human CD3 antibody, HIT3a clone, Biolegend, cat.300328; APC-Cy7 anti-human CD4 antibody, OKT4 clone, Biolegend, cat. 317418; Anti-CD3 monoclonal antibody, OKT3 clone, ThermoFisher, cat. 16-0037-81; APC anti-human CD4, RPA-T4 clone, Biolegend, cat. 300514; Pacific blue anti-human CD8, SK1 clone, Biolegend, cat. 344718; APC anti-human CD45RA, HI100 clone, Biolegend, cat. 304112; PE-Cy7 anti-human HLA-DR, L243 clone, Biolegend, cat. 307616; CellTraceTM CFSE Cell Proliferation Kit, for flow cytometry, ThermoFisher, cat. C34554; CD45RA MicroBeads, human, Miltenyi Biotec, cat. 130-045-901; ELISA MAXTM Standard Set Human IFN-γ, Biolegend, cat. 430101; LEGENDplex Human T Helper Cytokine Panels, Biolegend, cat. 740001; LIVE/DEADTM Fixable Yellow Dead Cell Stain Kit, for 405 nm excitation, ThermoFisher, cat. L34968; LIVE/DEADTM Fixable Aqua Dead Cell Stain Kit, for 405 nm excitation, ThermoFisher, cat. L34957; Human GM-CSF DuoSet ELISA, rndsystems, cat. DY215; Human XL Cytokine Luminex® Performance Assay 44-plex Fixed Panel, rndsystems,cat. ..

    Recombinase Polymerase Amplification:

    Article Title: Microbial peptides activate tumour-infiltrating lymphocytes in glioblastoma
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used PerCP/Cy5.5 anti-human CD3 antibody, HIT3a clone, Biolegend, cat.300328; APC-Cy7 anti-human CD4 antibody, OKT4 clone, Biolegend, cat. 317418; Anti-CD3 monoclonal antibody, OKT3 clone, ThermoFisher, cat. 16-0037-81; APC anti-human CD4, RPA-T4 clone, Biolegend, cat. 300514; Pacific blue anti-human CD8, SK1 clone, Biolegend, cat. 344718; APC anti-human CD45RA, HI100 clone, Biolegend, cat. 304112; PE-Cy7 anti-human HLA-DR, L243 clone, Biolegend, cat. 307616; CellTraceTM CFSE Cell Proliferation Kit, for flow cytometry, ThermoFisher, cat. C34554; CD45RA MicroBeads, human, Miltenyi Biotec, cat. 130-045-901; ELISA MAXTM Standard Set Human IFN-γ, Biolegend, cat. 430101; LEGENDplex Human T Helper Cytokine Panels, Biolegend, cat. 740001; LIVE/DEADTM Fixable Yellow Dead Cell Stain Kit, for 405 nm excitation, ThermoFisher, cat. L34968; LIVE/DEADTM Fixable Aqua Dead Cell Stain Kit, for 405 nm excitation, ThermoFisher, cat. L34957; Human GM-CSF DuoSet ELISA, rndsystems, cat. DY215; Human XL Cytokine Luminex® Performance Assay 44-plex Fixed Panel, rndsystems,cat. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Microbial peptides activate tumour-infiltrating lymphocytes in glioblastoma
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used PerCP/Cy5.5 anti-human CD3 antibody, HIT3a clone, Biolegend, cat.300328; APC-Cy7 anti-human CD4 antibody, OKT4 clone, Biolegend, cat. 317418; Anti-CD3 monoclonal antibody, OKT3 clone, ThermoFisher, cat. 16-0037-81; APC anti-human CD4, RPA-T4 clone, Biolegend, cat. 300514; Pacific blue anti-human CD8, SK1 clone, Biolegend, cat. 344718; APC anti-human CD45RA, HI100 clone, Biolegend, cat. 304112; PE-Cy7 anti-human HLA-DR, L243 clone, Biolegend, cat. 307616; CellTraceTM CFSE Cell Proliferation Kit, for flow cytometry, ThermoFisher, cat. C34554; CD45RA MicroBeads, human, Miltenyi Biotec, cat. 130-045-901; ELISA MAXTM Standard Set Human IFN-γ, Biolegend, cat. 430101; LEGENDplex Human T Helper Cytokine Panels, Biolegend, cat. 740001; LIVE/DEADTM Fixable Yellow Dead Cell Stain Kit, for 405 nm excitation, ThermoFisher, cat. L34968; LIVE/DEADTM Fixable Aqua Dead Cell Stain Kit, for 405 nm excitation, ThermoFisher, cat. L34957; Human GM-CSF DuoSet ELISA, rndsystems, cat. DY215; Human XL Cytokine Luminex® Performance Assay 44-plex Fixed Panel, rndsystems,cat. ..

    Staining:

    Article Title: Microbial peptides activate tumour-infiltrating lymphocytes in glioblastoma
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used PerCP/Cy5.5 anti-human CD3 antibody, HIT3a clone, Biolegend, cat.300328; APC-Cy7 anti-human CD4 antibody, OKT4 clone, Biolegend, cat. 317418; Anti-CD3 monoclonal antibody, OKT3 clone, ThermoFisher, cat. 16-0037-81; APC anti-human CD4, RPA-T4 clone, Biolegend, cat. 300514; Pacific blue anti-human CD8, SK1 clone, Biolegend, cat. 344718; APC anti-human CD45RA, HI100 clone, Biolegend, cat. 304112; PE-Cy7 anti-human HLA-DR, L243 clone, Biolegend, cat. 307616; CellTraceTM CFSE Cell Proliferation Kit, for flow cytometry, ThermoFisher, cat. C34554; CD45RA MicroBeads, human, Miltenyi Biotec, cat. 130-045-901; ELISA MAXTM Standard Set Human IFN-γ, Biolegend, cat. 430101; LEGENDplex Human T Helper Cytokine Panels, Biolegend, cat. 740001; LIVE/DEADTM Fixable Yellow Dead Cell Stain Kit, for 405 nm excitation, ThermoFisher, cat. L34968; LIVE/DEADTM Fixable Aqua Dead Cell Stain Kit, for 405 nm excitation, ThermoFisher, cat. L34957; Human GM-CSF DuoSet ELISA, rndsystems, cat. DY215; Human XL Cytokine Luminex® Performance Assay 44-plex Fixed Panel, rndsystems,cat. ..

    FACS:

    Article Title: The Master Regulator protein BAZ2B can reprogram human hematopoietic lineage-committed progenitors into a multipotent state.
    Article Snippet: .. For the FACS analysis of FISC and MPP populations in our cell culture experiments, we used the following combination of antibodies – Alexa Fluor 700 anti-human CD34 (clone 581), PE-Cy7 anti-human CD38 (clone HB7), APC anti-human CD45RA (clone HI100), PE anti-human CD90 (clone 5E10), and antibody and a biotin-labeled cocktail of antibodies (from Miltenyi) targeting the human “Lineage” antigens CD2, CD3, CD11b, CD14, CD15, CD16, CD19, CD56, CD123, and CD235a. .. For the primary colony-forming cell (CFC) assays, the 2000 FACS-sorted HSPCs were plated in Human Methocult Classic (H4434, Stemcell Technologies) on 35 mm plates and cultured for 14 days at 37 degrees C before the enumeration of colonies.

    Article Title: The Master Regulator protein BAZ2B can reprogram human hematopoietic lineage-committed progenitors into a multipotent state.
    Article Snippet: The cells were washed again and then incubated with Alexa Fluor 568 Phalloidin at a dilution of 1:40 for 20 mins at room temperature. .. Antibody panel for marking the HSCs and MPPs For the FACS analysis of FISC and MPP populations in our cell culture experiments, we used the following combination of antibodies – Alexa Fluor 700 anti-human CD34 (clone 581), PE-Cy7 anti-human CD38 (clone HB7), APC anti-human CD45RA (clone HI100), PE anti-human CD90 (clone 5E10), and antibody and a biotin-labeled cocktail of antibodies (from Miltenyi) targeting the human “Lineage” antigens CD2, CD3, CD11b, CD14, CD15, CD16, CD19, CD56, CD123, and CD235a. .. Confocal imaging was performed on a Leica TCS SPE inverted confocal microscope.

    Article Title: Method of generating multipotent stem cells
    Article Snippet: .. For the FACS analysis of HSC and MPP populations in our cell culture experiments, we used the following combination of antibodies—Alexa Fluor 700 anti-human CD34 (clone 581), PE-Cy7 anti-human CD38 (clone HB7), APC anti-human CD45RA (clone HI100), PE anti-human CD90 (clone 5E10), and antibody and a biotin-labeled cocktail of antibodies (from Miltenyi®) targeting the “Lineage” antigens CD2, CD3, CD11b, CD14, CD15, CD16, CD19, CD56, CD123, and CD235a. .. For the primary colony-forming cell (CFC) assays, the 2000 FACS-sorted HSPCs were plated in Human Methocult (H4434, StemCell Technologies®) on 35 mm plates and cultured for 14 days at 37° C. before the enumeration of colonies.

    Cell Culture:

    Article Title: The Master Regulator protein BAZ2B can reprogram human hematopoietic lineage-committed progenitors into a multipotent state.
    Article Snippet: .. For the FACS analysis of FISC and MPP populations in our cell culture experiments, we used the following combination of antibodies – Alexa Fluor 700 anti-human CD34 (clone 581), PE-Cy7 anti-human CD38 (clone HB7), APC anti-human CD45RA (clone HI100), PE anti-human CD90 (clone 5E10), and antibody and a biotin-labeled cocktail of antibodies (from Miltenyi) targeting the human “Lineage” antigens CD2, CD3, CD11b, CD14, CD15, CD16, CD19, CD56, CD123, and CD235a. .. For the primary colony-forming cell (CFC) assays, the 2000 FACS-sorted HSPCs were plated in Human Methocult Classic (H4434, Stemcell Technologies) on 35 mm plates and cultured for 14 days at 37 degrees C before the enumeration of colonies.

    Article Title: The Master Regulator protein BAZ2B can reprogram human hematopoietic lineage-committed progenitors into a multipotent state.
    Article Snippet: The cells were washed again and then incubated with Alexa Fluor 568 Phalloidin at a dilution of 1:40 for 20 mins at room temperature. .. Antibody panel for marking the HSCs and MPPs For the FACS analysis of FISC and MPP populations in our cell culture experiments, we used the following combination of antibodies – Alexa Fluor 700 anti-human CD34 (clone 581), PE-Cy7 anti-human CD38 (clone HB7), APC anti-human CD45RA (clone HI100), PE anti-human CD90 (clone 5E10), and antibody and a biotin-labeled cocktail of antibodies (from Miltenyi) targeting the human “Lineage” antigens CD2, CD3, CD11b, CD14, CD15, CD16, CD19, CD56, CD123, and CD235a. .. Confocal imaging was performed on a Leica TCS SPE inverted confocal microscope.

    Article Title: Method of generating multipotent stem cells
    Article Snippet: .. For the FACS analysis of HSC and MPP populations in our cell culture experiments, we used the following combination of antibodies—Alexa Fluor 700 anti-human CD34 (clone 581), PE-Cy7 anti-human CD38 (clone HB7), APC anti-human CD45RA (clone HI100), PE anti-human CD90 (clone 5E10), and antibody and a biotin-labeled cocktail of antibodies (from Miltenyi®) targeting the “Lineage” antigens CD2, CD3, CD11b, CD14, CD15, CD16, CD19, CD56, CD123, and CD235a. .. For the primary colony-forming cell (CFC) assays, the 2000 FACS-sorted HSPCs were plated in Human Methocult (H4434, StemCell Technologies®) on 35 mm plates and cultured for 14 days at 37° C. before the enumeration of colonies.



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    Anti Human Cd45ra Apc, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    (A) Schematic description of naïve CD4 T-cell differentiation towards FOXP3-expressing regulatory T cells and RORƔt-expressing T H 17 cells, including respective cytokine polarization milieus. (B, C) Percentages of CD45RA − cells and (C) CD25 + CD127 lo cells upon polarization of naïve CD4 T cells toward iT reg cells in various NS8593 concentrations (red) compared with DMSO control (Ctrl, black), n = 6–7. (D, E) Representative FACS histograms and (E) quantification of FOXP3 expression levels of CD25 + CD127 lo iT reg cells upon 6 d polarization of naïve CD4 T cells in presence of various NS8593 concentrations (red) or DMSO control (Ctrl, black), n = 6–7. (F, G) Percentages of CD45RA − cells and (G) CD25 + CD127 lo cells upon polarization of naïve CD4 T cells toward iT reg cells in presence of 6 mM MgCl 2 (MgCl 2 , blue) compared with H 2 O control (Ctrl, black), n = 7. (H, I) Representative FACS histograms and (I) quantification of FOXP3 expression levels of CD25 + CD127 lo iT reg cells upon 6 d polarization of naïve CD4 T cells in presence of 6 mM MgCl 2 (MgCl 2 , blue) compared with H 2 O control (Ctrl, black), n = 6. (J) Percentages of CCR6 + cells upon polarization of naïve CD4 T cells towards iT H 17 cells in presence of various NS8593 concentrations (red) compared with DMSO control (Ctrl, black), n = 6. (K, L) Representative FACS histograms and (L) quantification of RORƔt expression levels of CCR6 + iT H 17 cells upon 6 d polarization of naïve CD4 T cells in presence of various NS8593 concentrations (red) or DMSO control (Ctrl, black), n = 4–6. (M) Percentages of CCR6 + cells upon polarization of naïve CD4 T cells towards iT H 17 cells in presence of 6 mM MgCl 2 (MgCl 2 , blue) compared with H 2 O control (Ctrl, black), n = 6. (N, O) Representative FACS histograms and (O) quantification of RORƔt expression levels of CCR6 + iT H 17 cells upon 6 d polarization of naïve CD4 T cells in presence of 6 mM MgCl 2 (MgCl 2 , blue) compared with H 2 O control (Ctrl, black), n = 5. (P) Graphical summary of TRPM7-(in)dependent T-cell activation and differentiation towards iT reg and iT H 17 cells. Pharmacological blockade of TRPM7 reduces intracellular Mg 2+ levels, leads to reduced Ca 2+ signaling and results in reduced IL-2 secretion, impaired up-regulation of T-cell activation markers CD69 and CD25, and diminished proliferation upon TCR stimulus (left). TRPM7 inhibition during polarization of naïve CD4 T cells into iT reg cells preserves FOXP3 + signals of CD25 + CD127 lo iT reg cells. Polarization of naïve CD4 T cells into iT H 17 cells results in augmented RORƔt expression in the presence of 6 mM Mg 2+ , which is reduced upon TRPM7 inhibition, highlighting the need for Mg 2+ uptake and related TRPM7-dependent intracellular signaling for iT H 17 cell polarization (right). (B, C, E, F, G, I, J, L, M, O) Statistics: one-way ANOVA (B, C, E, J, L) and t test (F, G, I, M, O). * P < 0.05; ** P < 0.005; *** P < 0.0005; **** P < 0.0001 and n.s., not significant. Data are mean ± SD.

    Journal: Life Science Alliance

    Article Title: TRPM7 and magnesium orchestrate human CD4 T-cell activation and differentiation

    doi: 10.26508/lsa.202503357

    Figure Lengend Snippet: (A) Schematic description of naïve CD4 T-cell differentiation towards FOXP3-expressing regulatory T cells and RORƔt-expressing T H 17 cells, including respective cytokine polarization milieus. (B, C) Percentages of CD45RA − cells and (C) CD25 + CD127 lo cells upon polarization of naïve CD4 T cells toward iT reg cells in various NS8593 concentrations (red) compared with DMSO control (Ctrl, black), n = 6–7. (D, E) Representative FACS histograms and (E) quantification of FOXP3 expression levels of CD25 + CD127 lo iT reg cells upon 6 d polarization of naïve CD4 T cells in presence of various NS8593 concentrations (red) or DMSO control (Ctrl, black), n = 6–7. (F, G) Percentages of CD45RA − cells and (G) CD25 + CD127 lo cells upon polarization of naïve CD4 T cells toward iT reg cells in presence of 6 mM MgCl 2 (MgCl 2 , blue) compared with H 2 O control (Ctrl, black), n = 7. (H, I) Representative FACS histograms and (I) quantification of FOXP3 expression levels of CD25 + CD127 lo iT reg cells upon 6 d polarization of naïve CD4 T cells in presence of 6 mM MgCl 2 (MgCl 2 , blue) compared with H 2 O control (Ctrl, black), n = 6. (J) Percentages of CCR6 + cells upon polarization of naïve CD4 T cells towards iT H 17 cells in presence of various NS8593 concentrations (red) compared with DMSO control (Ctrl, black), n = 6. (K, L) Representative FACS histograms and (L) quantification of RORƔt expression levels of CCR6 + iT H 17 cells upon 6 d polarization of naïve CD4 T cells in presence of various NS8593 concentrations (red) or DMSO control (Ctrl, black), n = 4–6. (M) Percentages of CCR6 + cells upon polarization of naïve CD4 T cells towards iT H 17 cells in presence of 6 mM MgCl 2 (MgCl 2 , blue) compared with H 2 O control (Ctrl, black), n = 6. (N, O) Representative FACS histograms and (O) quantification of RORƔt expression levels of CCR6 + iT H 17 cells upon 6 d polarization of naïve CD4 T cells in presence of 6 mM MgCl 2 (MgCl 2 , blue) compared with H 2 O control (Ctrl, black), n = 5. (P) Graphical summary of TRPM7-(in)dependent T-cell activation and differentiation towards iT reg and iT H 17 cells. Pharmacological blockade of TRPM7 reduces intracellular Mg 2+ levels, leads to reduced Ca 2+ signaling and results in reduced IL-2 secretion, impaired up-regulation of T-cell activation markers CD69 and CD25, and diminished proliferation upon TCR stimulus (left). TRPM7 inhibition during polarization of naïve CD4 T cells into iT reg cells preserves FOXP3 + signals of CD25 + CD127 lo iT reg cells. Polarization of naïve CD4 T cells into iT H 17 cells results in augmented RORƔt expression in the presence of 6 mM Mg 2+ , which is reduced upon TRPM7 inhibition, highlighting the need for Mg 2+ uptake and related TRPM7-dependent intracellular signaling for iT H 17 cell polarization (right). (B, C, E, F, G, I, J, L, M, O) Statistics: one-way ANOVA (B, C, E, J, L) and t test (F, G, I, M, O). * P < 0.05; ** P < 0.005; *** P < 0.0005; **** P < 0.0001 and n.s., not significant. Data are mean ± SD.

    Article Snippet: The following antibodies were used: α-human CD4-VioBlue (REA623; Miltenyi), α-human CD45RA-APC-Vio770 (REA562; Miltenyi), α-human CD69-APC (REA824; Miltenyi), α-human CD25-VioBright515 (REA570; Miltenyi).

    Techniques: Cell Differentiation, Expressing, Control, Activation Assay, Inhibition

    (A) Representative FACS plots and gating strategy for iT reg cells after 6 d of differentiation of naïve CD4 T cells, shown for Ctrl cells. Naïve CD4 T cells were used for gating. (B) Quantification of FOXP3 expression in CD25 + CD127 lo cells of Apamin treated cells (Apamin, blue) compared with DMSO control (Ctrl, gray), gated as in (A), n = 6. (C) Representative FACS plots and gating strategy for iT reg cells after 6 d of differentiation in presence of various Waixenicin A concentrations (WxA) compared with EtOH control (Ctrl). Naïve CD4 T cells were used for gating. (D, E, F) Percentage of CD45RA + (D), CD45RA − (E) and CD25 + CD127 lo (F) cells upon polarization of naïve CD4 T cells towards T reg cells in presence of various Waixenicin A concentrations (WxA, green) compared with EtOH control (Ctrl, black), gated as in (C), n = 4. (B, D, E, F) Statistics: t test (B) and one-way ANOVA (D, E, F). * P < 0.05, ** P < 0.005, **** P < 0.0001 and n.s., not significant. Data are mean ± SD.

    Journal: Life Science Alliance

    Article Title: TRPM7 and magnesium orchestrate human CD4 T-cell activation and differentiation

    doi: 10.26508/lsa.202503357

    Figure Lengend Snippet: (A) Representative FACS plots and gating strategy for iT reg cells after 6 d of differentiation of naïve CD4 T cells, shown for Ctrl cells. Naïve CD4 T cells were used for gating. (B) Quantification of FOXP3 expression in CD25 + CD127 lo cells of Apamin treated cells (Apamin, blue) compared with DMSO control (Ctrl, gray), gated as in (A), n = 6. (C) Representative FACS plots and gating strategy for iT reg cells after 6 d of differentiation in presence of various Waixenicin A concentrations (WxA) compared with EtOH control (Ctrl). Naïve CD4 T cells were used for gating. (D, E, F) Percentage of CD45RA + (D), CD45RA − (E) and CD25 + CD127 lo (F) cells upon polarization of naïve CD4 T cells towards T reg cells in presence of various Waixenicin A concentrations (WxA, green) compared with EtOH control (Ctrl, black), gated as in (C), n = 4. (B, D, E, F) Statistics: t test (B) and one-way ANOVA (D, E, F). * P < 0.05, ** P < 0.005, **** P < 0.0001 and n.s., not significant. Data are mean ± SD.

    Article Snippet: The following antibodies were used: α-human CD4-VioBlue (REA623; Miltenyi), α-human CD45RA-APC-Vio770 (REA562; Miltenyi), α-human CD69-APC (REA824; Miltenyi), α-human CD25-VioBright515 (REA570; Miltenyi).

    Techniques: Expressing, Control