Chromatin Immunoprecipitation:Article Title: Microbial peptides activate tumour-infiltrating lymphocytes in glioblastoma
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used PerCP/Cy5.5 anti-human CD3 antibody, HIT3a clone, Biolegend, cat.300328; APC-Cy7 anti-human CD4 antibody, OKT4 clone, Biolegend, cat. 317418; Anti-CD3 monoclonal antibody, OKT3 clone, ThermoFisher, cat. 16-0037-81; APC anti-human CD4, RPA-T4 clone, Biolegend, cat. 300514; Pacific blue anti-human CD8, SK1 clone, Biolegend, cat. 344718; APC anti-human CD45RA, HI100 clone, Biolegend, cat. 304112; PE-Cy7 anti-human HLA-DR, L243 clone, Biolegend, cat. 307616; CellTraceTM CFSE Cell Proliferation Kit, for flow cytometry, ThermoFisher, cat. C34554; CD45RA MicroBeads, human, Miltenyi Biotec, cat. 130-045-901; ELISA MAXTM Standard Set Human IFN-γ, Biolegend, cat. 430101; LEGENDplex Human T Helper Cytokine Panels, Biolegend, cat. 740001; LIVE/DEADTM Fixable Yellow Dead Cell Stain Kit, for 405 nm excitation, ThermoFisher, cat. L34968; LIVE/DEADTM Fixable Aqua Dead Cell Stain Kit, for 405 nm excitation, ThermoFisher, cat. L34957; Human GM-CSF DuoSet ELISA, rndsystems, cat. DY215; Human XL Cytokine Luminex® Performance Assay 44-plex Fixed Panel, rndsystems,cat. ..
Flow Cytometry:Article Title: Microbial peptides activate tumour-infiltrating lymphocytes in glioblastoma
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used PerCP/Cy5.5 anti-human CD3 antibody, HIT3a clone, Biolegend, cat.300328; APC-Cy7 anti-human CD4 antibody, OKT4 clone, Biolegend, cat. 317418; Anti-CD3 monoclonal antibody, OKT3 clone, ThermoFisher, cat. 16-0037-81; APC anti-human CD4, RPA-T4 clone, Biolegend, cat. 300514; Pacific blue anti-human CD8, SK1 clone, Biolegend, cat. 344718; APC anti-human CD45RA, HI100 clone, Biolegend, cat. 304112; PE-Cy7 anti-human HLA-DR, L243 clone, Biolegend, cat. 307616; CellTraceTM CFSE Cell Proliferation Kit, for flow cytometry, ThermoFisher, cat. C34554; CD45RA MicroBeads, human, Miltenyi Biotec, cat. 130-045-901; ELISA MAXTM Standard Set Human IFN-γ, Biolegend, cat. 430101; LEGENDplex Human T Helper Cytokine Panels, Biolegend, cat. 740001; LIVE/DEADTM Fixable Yellow Dead Cell Stain Kit, for 405 nm excitation, ThermoFisher, cat. L34968; LIVE/DEADTM Fixable Aqua Dead Cell Stain Kit, for 405 nm excitation, ThermoFisher, cat. L34957; Human GM-CSF DuoSet ELISA, rndsystems, cat. DY215; Human XL Cytokine Luminex® Performance Assay 44-plex Fixed Panel, rndsystems,cat. ..
Magnetic Resonance Imaging:Article Title: Microbial peptides activate tumour-infiltrating lymphocytes in glioblastoma
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used PerCP/Cy5.5 anti-human CD3 antibody, HIT3a clone, Biolegend, cat.300328; APC-Cy7 anti-human CD4 antibody, OKT4 clone, Biolegend, cat. 317418; Anti-CD3 monoclonal antibody, OKT3 clone, ThermoFisher, cat. 16-0037-81; APC anti-human CD4, RPA-T4 clone, Biolegend, cat. 300514; Pacific blue anti-human CD8, SK1 clone, Biolegend, cat. 344718; APC anti-human CD45RA, HI100 clone, Biolegend, cat. 304112; PE-Cy7 anti-human HLA-DR, L243 clone, Biolegend, cat. 307616; CellTraceTM CFSE Cell Proliferation Kit, for flow cytometry, ThermoFisher, cat. C34554; CD45RA MicroBeads, human, Miltenyi Biotec, cat. 130-045-901; ELISA MAXTM Standard Set Human IFN-γ, Biolegend, cat. 430101; LEGENDplex Human T Helper Cytokine Panels, Biolegend, cat. 740001; LIVE/DEADTM Fixable Yellow Dead Cell Stain Kit, for 405 nm excitation, ThermoFisher, cat. L34968; LIVE/DEADTM Fixable Aqua Dead Cell Stain Kit, for 405 nm excitation, ThermoFisher, cat. L34957; Human GM-CSF DuoSet ELISA, rndsystems, cat. DY215; Human XL Cytokine Luminex® Performance Assay 44-plex Fixed Panel, rndsystems,cat. ..
Recombinase Polymerase Amplification:Article Title: Microbial peptides activate tumour-infiltrating lymphocytes in glioblastoma
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used PerCP/Cy5.5 anti-human CD3 antibody, HIT3a clone, Biolegend, cat.300328; APC-Cy7 anti-human CD4 antibody, OKT4 clone, Biolegend, cat. 317418; Anti-CD3 monoclonal antibody, OKT3 clone, ThermoFisher, cat. 16-0037-81; APC anti-human CD4, RPA-T4 clone, Biolegend, cat. 300514; Pacific blue anti-human CD8, SK1 clone, Biolegend, cat. 344718; APC anti-human CD45RA, HI100 clone, Biolegend, cat. 304112; PE-Cy7 anti-human HLA-DR, L243 clone, Biolegend, cat. 307616; CellTraceTM CFSE Cell Proliferation Kit, for flow cytometry, ThermoFisher, cat. C34554; CD45RA MicroBeads, human, Miltenyi Biotec, cat. 130-045-901; ELISA MAXTM Standard Set Human IFN-γ, Biolegend, cat. 430101; LEGENDplex Human T Helper Cytokine Panels, Biolegend, cat. 740001; LIVE/DEADTM Fixable Yellow Dead Cell Stain Kit, for 405 nm excitation, ThermoFisher, cat. L34968; LIVE/DEADTM Fixable Aqua Dead Cell Stain Kit, for 405 nm excitation, ThermoFisher, cat. L34957; Human GM-CSF DuoSet ELISA, rndsystems, cat. DY215; Human XL Cytokine Luminex® Performance Assay 44-plex Fixed Panel, rndsystems,cat. ..
Enzyme-linked Immunosorbent Assay:Article Title: Microbial peptides activate tumour-infiltrating lymphocytes in glioblastoma
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used PerCP/Cy5.5 anti-human CD3 antibody, HIT3a clone, Biolegend, cat.300328; APC-Cy7 anti-human CD4 antibody, OKT4 clone, Biolegend, cat. 317418; Anti-CD3 monoclonal antibody, OKT3 clone, ThermoFisher, cat. 16-0037-81; APC anti-human CD4, RPA-T4 clone, Biolegend, cat. 300514; Pacific blue anti-human CD8, SK1 clone, Biolegend, cat. 344718; APC anti-human CD45RA, HI100 clone, Biolegend, cat. 304112; PE-Cy7 anti-human HLA-DR, L243 clone, Biolegend, cat. 307616; CellTraceTM CFSE Cell Proliferation Kit, for flow cytometry, ThermoFisher, cat. C34554; CD45RA MicroBeads, human, Miltenyi Biotec, cat. 130-045-901; ELISA MAXTM Standard Set Human IFN-γ, Biolegend, cat. 430101; LEGENDplex Human T Helper Cytokine Panels, Biolegend, cat. 740001; LIVE/DEADTM Fixable Yellow Dead Cell Stain Kit, for 405 nm excitation, ThermoFisher, cat. L34968; LIVE/DEADTM Fixable Aqua Dead Cell Stain Kit, for 405 nm excitation, ThermoFisher, cat. L34957; Human GM-CSF DuoSet ELISA, rndsystems, cat. DY215; Human XL Cytokine Luminex® Performance Assay 44-plex Fixed Panel, rndsystems,cat. ..
Staining:Article Title: Microbial peptides activate tumour-infiltrating lymphocytes in glioblastoma
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used PerCP/Cy5.5 anti-human CD3 antibody, HIT3a clone, Biolegend, cat.300328; APC-Cy7 anti-human CD4 antibody, OKT4 clone, Biolegend, cat. 317418; Anti-CD3 monoclonal antibody, OKT3 clone, ThermoFisher, cat. 16-0037-81; APC anti-human CD4, RPA-T4 clone, Biolegend, cat. 300514; Pacific blue anti-human CD8, SK1 clone, Biolegend, cat. 344718; APC anti-human CD45RA, HI100 clone, Biolegend, cat. 304112; PE-Cy7 anti-human HLA-DR, L243 clone, Biolegend, cat. 307616; CellTraceTM CFSE Cell Proliferation Kit, for flow cytometry, ThermoFisher, cat. C34554; CD45RA MicroBeads, human, Miltenyi Biotec, cat. 130-045-901; ELISA MAXTM Standard Set Human IFN-γ, Biolegend, cat. 430101; LEGENDplex Human T Helper Cytokine Panels, Biolegend, cat. 740001; LIVE/DEADTM Fixable Yellow Dead Cell Stain Kit, for 405 nm excitation, ThermoFisher, cat. L34968; LIVE/DEADTM Fixable Aqua Dead Cell Stain Kit, for 405 nm excitation, ThermoFisher, cat. L34957; Human GM-CSF DuoSet ELISA, rndsystems, cat. DY215; Human XL Cytokine Luminex® Performance Assay 44-plex Fixed Panel, rndsystems,cat. ..
FACS:Article Title: The Master Regulator protein BAZ2B can reprogram human hematopoietic lineage-committed progenitors into a multipotent state.
Article Snippet: .. For the FACS analysis of FISC and MPP populations in our cell culture experiments, we used the following combination of antibodies – Alexa Fluor 700 anti-human CD34 (clone 581), PE-Cy7 anti-human CD38 (clone HB7), APC anti-human CD45RA (clone HI100), PE anti-human CD90 (clone 5E10), and antibody and a biotin-labeled cocktail of antibodies (from Miltenyi) targeting the human “Lineage” antigens CD2, CD3, CD11b, CD14, CD15, CD16, CD19, CD56, CD123, and CD235a. .. For the primary colony-forming cell (CFC) assays, the 2000 FACS-sorted HSPCs were plated in Human Methocult Classic (H4434, Stemcell Technologies) on 35 mm plates and cultured for 14 days at 37 degrees C before the enumeration of colonies.
Article Title: The Master Regulator protein BAZ2B can reprogram human hematopoietic lineage-committed progenitors into a multipotent state.
Article Snippet: The cells were washed again and then incubated with Alexa Fluor 568 Phalloidin at a dilution of 1:40 for 20 mins at room temperature. .. Antibody panel for marking the HSCs and MPPs For the FACS analysis of FISC and MPP populations in our cell culture experiments, we used the following combination of antibodies – Alexa Fluor 700 anti-human CD34 (clone 581), PE-Cy7 anti-human CD38 (clone HB7), APC anti-human CD45RA (clone HI100), PE anti-human CD90 (clone 5E10), and antibody and a biotin-labeled cocktail of antibodies (from Miltenyi) targeting the human “Lineage” antigens CD2, CD3, CD11b, CD14, CD15, CD16, CD19, CD56, CD123, and CD235a. .. Confocal imaging was performed on a Leica TCS SPE inverted confocal microscope.
Article Title: Method of generating multipotent stem cells
Article Snippet: .. For the FACS analysis of HSC and MPP populations in our cell culture experiments, we used the following combination of antibodies—Alexa Fluor 700 anti-human CD34 (clone 581), PE-Cy7 anti-human CD38 (clone HB7), APC anti-human CD45RA (clone HI100), PE anti-human CD90 (clone 5E10), and antibody and a biotin-labeled cocktail of antibodies (from Miltenyi®) targeting the “Lineage” antigens CD2, CD3, CD11b, CD14, CD15, CD16, CD19, CD56, CD123, and CD235a. .. For the primary colony-forming cell (CFC) assays, the 2000 FACS-sorted HSPCs were plated in Human Methocult (H4434, StemCell Technologies®) on 35 mm plates and cultured for 14 days at 37° C. before the enumeration of colonies.
Cell Culture:Article Title: The Master Regulator protein BAZ2B can reprogram human hematopoietic lineage-committed progenitors into a multipotent state.
Article Snippet: .. For the FACS analysis of FISC and MPP populations in our cell culture experiments, we used the following combination of antibodies – Alexa Fluor 700 anti-human CD34 (clone 581), PE-Cy7 anti-human CD38 (clone HB7), APC anti-human CD45RA (clone HI100), PE anti-human CD90 (clone 5E10), and antibody and a biotin-labeled cocktail of antibodies (from Miltenyi) targeting the human “Lineage” antigens CD2, CD3, CD11b, CD14, CD15, CD16, CD19, CD56, CD123, and CD235a. .. For the primary colony-forming cell (CFC) assays, the 2000 FACS-sorted HSPCs were plated in Human Methocult Classic (H4434, Stemcell Technologies) on 35 mm plates and cultured for 14 days at 37 degrees C before the enumeration of colonies.
Article Title: The Master Regulator protein BAZ2B can reprogram human hematopoietic lineage-committed progenitors into a multipotent state.
Article Snippet: The cells were washed again and then incubated with Alexa Fluor 568 Phalloidin at a dilution of 1:40 for 20 mins at room temperature. .. Antibody panel for marking the HSCs and MPPs For the FACS analysis of FISC and MPP populations in our cell culture experiments, we used the following combination of antibodies – Alexa Fluor 700 anti-human CD34 (clone 581), PE-Cy7 anti-human CD38 (clone HB7), APC anti-human CD45RA (clone HI100), PE anti-human CD90 (clone 5E10), and antibody and a biotin-labeled cocktail of antibodies (from Miltenyi) targeting the human “Lineage” antigens CD2, CD3, CD11b, CD14, CD15, CD16, CD19, CD56, CD123, and CD235a. .. Confocal imaging was performed on a Leica TCS SPE inverted confocal microscope.
Article Title: Method of generating multipotent stem cells
Article Snippet: .. For the FACS analysis of HSC and MPP populations in our cell culture experiments, we used the following combination of antibodies—Alexa Fluor 700 anti-human CD34 (clone 581), PE-Cy7 anti-human CD38 (clone HB7), APC anti-human CD45RA (clone HI100), PE anti-human CD90 (clone 5E10), and antibody and a biotin-labeled cocktail of antibodies (from Miltenyi®) targeting the “Lineage” antigens CD2, CD3, CD11b, CD14, CD15, CD16, CD19, CD56, CD123, and CD235a. .. For the primary colony-forming cell (CFC) assays, the 2000 FACS-sorted HSPCs were plated in Human Methocult (H4434, StemCell Technologies®) on 35 mm plates and cultured for 14 days at 37° C. before the enumeration of colonies.
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